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ulex europaeus agglutinin 1 lectin conjugated to biotin  (Vector Laboratories)


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    Vector Laboratories ulex europaeus agglutinin 1 lectin conjugated to biotin
    Ulex Europaeus Agglutinin 1 Lectin Conjugated To Biotin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 472 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ulex europaeus agglutinin 1 lectin conjugated to biotin/product/Vector Laboratories
    Average 96 stars, based on 472 article reviews
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    FasLΔm/Δm mice had normal mTEC numbers with normal AIRE expression but loss of NF-κB2 caused abnormalities in the thymic stroma. (a) Representative confocal images of thymi from mice of the indicated genotypes at 6 weeks. Sections were stained for <t>UEA-1</t> (green), AIRE (red) and keratin-5 (blue); n=3/genotype/time point. (b) Representative confocal images of thymi from mice of the indicated genotypes at 20 weeks as for (a). (c) Graphical representation of total thymic cellularity from mice of the indicated genotypes at 20 weeks of age; n=4/genotype. (d) Graphical depiction of flow cytometric analysis of TECs from mice of the indicated genotypes at 20 weeks of age, as defined; mTEChi (CD45− Ep-CAM+class II MHChi Ly51−), mTEClow (CD45− Ep-CAM+class II MHClow Ly51−), cTEC (CD45− Ep-CAM+class II MHC+ Ly51+); n=4/genotype. (e) Graphical depiction of flow cytometric analysis of mTEChiAIRE+ mTECS (CD45− Ep-CAM+class II MHChi Ly51− AIRE+) from mice of the indicated genotypes at 20 weeks of age. (f) Percentages of thymocytes that are CD4 single positive (CD4+ CD8−) or CD8 single positive (CD4−CD8+) from mice of the indicated genotypes at 3 months of age; n=4/genotype. *P<0.05, **P<0.005
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    FasLΔm/Δm mice had normal mTEC numbers with normal AIRE expression but loss of NF-κB2 caused abnormalities in the thymic stroma. (a) Representative confocal images of thymi from mice of the indicated genotypes at 6 weeks. Sections were stained for <t>UEA-1</t> (green), AIRE (red) and keratin-5 (blue); n=3/genotype/time point. (b) Representative confocal images of thymi from mice of the indicated genotypes at 20 weeks as for (a). (c) Graphical representation of total thymic cellularity from mice of the indicated genotypes at 20 weeks of age; n=4/genotype. (d) Graphical depiction of flow cytometric analysis of TECs from mice of the indicated genotypes at 20 weeks of age, as defined; mTEChi (CD45− Ep-CAM+class II MHChi Ly51−), mTEClow (CD45− Ep-CAM+class II MHClow Ly51−), cTEC (CD45− Ep-CAM+class II MHC+ Ly51+); n=4/genotype. (e) Graphical depiction of flow cytometric analysis of mTEChiAIRE+ mTECS (CD45− Ep-CAM+class II MHChi Ly51− AIRE+) from mice of the indicated genotypes at 20 weeks of age. (f) Percentages of thymocytes that are CD4 single positive (CD4+ CD8−) or CD8 single positive (CD4−CD8+) from mice of the indicated genotypes at 3 months of age; n=4/genotype. *P<0.05, **P<0.005
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    FasLΔm/Δm mice had normal mTEC numbers with normal AIRE expression but loss of NF-κB2 caused abnormalities in the thymic stroma. (a) Representative confocal images of thymi from mice of the indicated genotypes at 6 weeks. Sections were stained for <t>UEA-1</t> (green), AIRE (red) and keratin-5 (blue); n=3/genotype/time point. (b) Representative confocal images of thymi from mice of the indicated genotypes at 20 weeks as for (a). (c) Graphical representation of total thymic cellularity from mice of the indicated genotypes at 20 weeks of age; n=4/genotype. (d) Graphical depiction of flow cytometric analysis of TECs from mice of the indicated genotypes at 20 weeks of age, as defined; mTEChi (CD45− Ep-CAM+class II MHChi Ly51−), mTEClow (CD45− Ep-CAM+class II MHClow Ly51−), cTEC (CD45− Ep-CAM+class II MHC+ Ly51+); n=4/genotype. (e) Graphical depiction of flow cytometric analysis of mTEChiAIRE+ mTECS (CD45− Ep-CAM+class II MHChi Ly51− AIRE+) from mice of the indicated genotypes at 20 weeks of age. (f) Percentages of thymocytes that are CD4 single positive (CD4+ CD8−) or CD8 single positive (CD4−CD8+) from mice of the indicated genotypes at 3 months of age; n=4/genotype. *P<0.05, **P<0.005
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    FasLΔm/Δm mice had normal mTEC numbers with normal AIRE expression but loss of NF-κB2 caused abnormalities in the thymic stroma. (a) Representative confocal images of thymi from mice of the indicated genotypes at 6 weeks. Sections were stained for <t>UEA-1</t> (green), AIRE (red) and keratin-5 (blue); n=3/genotype/time point. (b) Representative confocal images of thymi from mice of the indicated genotypes at 20 weeks as for (a). (c) Graphical representation of total thymic cellularity from mice of the indicated genotypes at 20 weeks of age; n=4/genotype. (d) Graphical depiction of flow cytometric analysis of TECs from mice of the indicated genotypes at 20 weeks of age, as defined; mTEChi (CD45− Ep-CAM+class II MHChi Ly51−), mTEClow (CD45− Ep-CAM+class II MHClow Ly51−), cTEC (CD45− Ep-CAM+class II MHC+ Ly51+); n=4/genotype. (e) Graphical depiction of flow cytometric analysis of mTEChiAIRE+ mTECS (CD45− Ep-CAM+class II MHChi Ly51− AIRE+) from mice of the indicated genotypes at 20 weeks of age. (f) Percentages of thymocytes that are CD4 single positive (CD4+ CD8−) or CD8 single positive (CD4−CD8+) from mice of the indicated genotypes at 3 months of age; n=4/genotype. *P<0.05, **P<0.005
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    FasLΔm/Δm mice had normal mTEC numbers with normal AIRE expression but loss of NF-κB2 caused abnormalities in the thymic stroma. (a) Representative confocal images of thymi from mice of the indicated genotypes at 6 weeks. Sections were stained for <t>UEA-1</t> (green), AIRE (red) and keratin-5 (blue); n=3/genotype/time point. (b) Representative confocal images of thymi from mice of the indicated genotypes at 20 weeks as for (a). (c) Graphical representation of total thymic cellularity from mice of the indicated genotypes at 20 weeks of age; n=4/genotype. (d) Graphical depiction of flow cytometric analysis of TECs from mice of the indicated genotypes at 20 weeks of age, as defined; mTEChi (CD45− Ep-CAM+class II MHChi Ly51−), mTEClow (CD45− Ep-CAM+class II MHClow Ly51−), cTEC (CD45− Ep-CAM+class II MHC+ Ly51+); n=4/genotype. (e) Graphical depiction of flow cytometric analysis of mTEChiAIRE+ mTECS (CD45− Ep-CAM+class II MHChi Ly51− AIRE+) from mice of the indicated genotypes at 20 weeks of age. (f) Percentages of thymocytes that are CD4 single positive (CD4+ CD8−) or CD8 single positive (CD4−CD8+) from mice of the indicated genotypes at 3 months of age; n=4/genotype. *P<0.05, **P<0.005
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    Average 95 stars, based on 1 article reviews
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    biotin-conjugated uea-1  (Vector Laboratories)
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    Vector Laboratories biotin-conjugated uea-1
    FasLΔm/Δm mice had normal mTEC numbers with normal AIRE expression but loss of NF-κB2 caused abnormalities in the thymic stroma. (a) Representative confocal images of thymi from mice of the indicated genotypes at 6 weeks. Sections were stained for <t>UEA-1</t> (green), AIRE (red) and keratin-5 (blue); n=3/genotype/time point. (b) Representative confocal images of thymi from mice of the indicated genotypes at 20 weeks as for (a). (c) Graphical representation of total thymic cellularity from mice of the indicated genotypes at 20 weeks of age; n=4/genotype. (d) Graphical depiction of flow cytometric analysis of TECs from mice of the indicated genotypes at 20 weeks of age, as defined; mTEChi (CD45− Ep-CAM+class II MHChi Ly51−), mTEClow (CD45− Ep-CAM+class II MHClow Ly51−), cTEC (CD45− Ep-CAM+class II MHC+ Ly51+); n=4/genotype. (e) Graphical depiction of flow cytometric analysis of mTEChiAIRE+ mTECS (CD45− Ep-CAM+class II MHChi Ly51− AIRE+) from mice of the indicated genotypes at 20 weeks of age. (f) Percentages of thymocytes that are CD4 single positive (CD4+ CD8−) or CD8 single positive (CD4−CD8+) from mice of the indicated genotypes at 3 months of age; n=4/genotype. *P<0.05, **P<0.005
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    FasLΔm/Δm mice had normal mTEC numbers with normal AIRE expression but loss of NF-κB2 caused abnormalities in the thymic stroma. (a) Representative confocal images of thymi from mice of the indicated genotypes at 6 weeks. Sections were stained for UEA-1 (green), AIRE (red) and keratin-5 (blue); n=3/genotype/time point. (b) Representative confocal images of thymi from mice of the indicated genotypes at 20 weeks as for (a). (c) Graphical representation of total thymic cellularity from mice of the indicated genotypes at 20 weeks of age; n=4/genotype. (d) Graphical depiction of flow cytometric analysis of TECs from mice of the indicated genotypes at 20 weeks of age, as defined; mTEChi (CD45− Ep-CAM+class II MHChi Ly51−), mTEClow (CD45− Ep-CAM+class II MHClow Ly51−), cTEC (CD45− Ep-CAM+class II MHC+ Ly51+); n=4/genotype. (e) Graphical depiction of flow cytometric analysis of mTEChiAIRE+ mTECS (CD45− Ep-CAM+class II MHChi Ly51− AIRE+) from mice of the indicated genotypes at 20 weeks of age. (f) Percentages of thymocytes that are CD4 single positive (CD4+ CD8−) or CD8 single positive (CD4−CD8+) from mice of the indicated genotypes at 3 months of age; n=4/genotype. *P<0.05, **P<0.005

    Journal: Cell Death and Differentiation

    Article Title: Loss of c-REL but not NF- κ B2 prevents autoimmune disease driven by FasL mutation

    doi: 10.1038/cdd.2014.168

    Figure Lengend Snippet: FasLΔm/Δm mice had normal mTEC numbers with normal AIRE expression but loss of NF-κB2 caused abnormalities in the thymic stroma. (a) Representative confocal images of thymi from mice of the indicated genotypes at 6 weeks. Sections were stained for UEA-1 (green), AIRE (red) and keratin-5 (blue); n=3/genotype/time point. (b) Representative confocal images of thymi from mice of the indicated genotypes at 20 weeks as for (a). (c) Graphical representation of total thymic cellularity from mice of the indicated genotypes at 20 weeks of age; n=4/genotype. (d) Graphical depiction of flow cytometric analysis of TECs from mice of the indicated genotypes at 20 weeks of age, as defined; mTEChi (CD45− Ep-CAM+class II MHChi Ly51−), mTEClow (CD45− Ep-CAM+class II MHClow Ly51−), cTEC (CD45− Ep-CAM+class II MHC+ Ly51+); n=4/genotype. (e) Graphical depiction of flow cytometric analysis of mTEChiAIRE+ mTECS (CD45− Ep-CAM+class II MHChi Ly51− AIRE+) from mice of the indicated genotypes at 20 weeks of age. (f) Percentages of thymocytes that are CD4 single positive (CD4+ CD8−) or CD8 single positive (CD4−CD8+) from mice of the indicated genotypes at 3 months of age; n=4/genotype. *P<0.05, **P<0.005

    Article Snippet: Primary Abs included biotin-conjugated anti-UEA-1 (Vector Laboratories, Burlingame, CA, USA), rabbit anti-keratin-5 (MK5, Covance, Biolegend, San Deigo, CA, USA) and Alexa 405-conjugated anti-AIRE.

    Techniques: Expressing, Staining